Gentamicin kills Gram-negative and Gram-positive bacteria, and
mycoplasma. It works by binding to microbial ribosomes and
preventing the initiation of protein synthesis. Recommended
dosing in media is 5mL/L.
Sterile-filtered
Ships on ice. Store refrigerated at 2 to 8°C
Amphotericin B is one of the earliest compounds designed to kill
fungi. It was developed to help treat fungal infections
intravenously. It has since been found to help eliminate fungal
contamination in cell cultures as well. Low doses (0.5 to 2.0
ug/mL) are recommended as it is toxic to other eukaryotic cells
at higher concentrations.
Final concentration of Amphotericin B is undiluted.
Sterile-filtered
Ships on dry ice. Store frozen at -20 to -10°C
FUNGIZONE® is a registered trademark of E.R. Squibb
This beta-lactam antibiotic is an aminopenicillin. Like
penicillin, it inhibits bacterial cell wall synthesis by
preventing peptidoglycan cross-linking and murein assembly. The
presence of an amino group confers greater efficacy against
Gram-negative bacteria as compared to penicillin. Ampicillin is
commonly used in molecular biology to assay for gene uptake
(e.g., plasmid vectors carrying the ampicillin resistance gene).
Gemini’s antibiotic:antimycotic (anti:anti) solution is for
general prophylactic use. Dilute 100-fold for use in media
(10mL/L). This product is useful as a broad spectrum
anti-bacterial and anti-fungal agent. This product is not
recommended for cultures with established fungal contamination
due to the low concentration of amphotericin B. See Amphotericin B for elimination of fungal
contamination in culture.
10,000 units/mL penicillin G, 10mg/mL streptomycin sulfate,
25µg/mL amphotericin B
Blasticidin, from Streptomyces griseochromogenes, is an
antibiotic commonly used in gene selection.
Blasticidin is active against both prokaryotic and eukaryotic.
Resistance to blasticidin is conferred by the blasticidin
resistance gene from bacillus cereus (bsr). Bsr codes for
blasticidin deaminase.
Carbenicillin is a third-generation analog of penicillin. Like
penicillin, it inhibits bacterial cell wall synthesis by
preventing peptidoglycan cross-linking and murein assembly. It
shows greater efficacy against Gram-negative bacteria as compared
to penicillin. It may be useful against some species of
Pseudomonas and Serratia, but has not been generally used in
mammalian cell cultures. However, plant cell culturists have
found this to be a useful prophylactic agent against many
organisms, such as Agrobacterium. Recommended concentration in
cell culture is 100 mg/L.
G418 sulfate, an aminoglycoside antibiotic, is related to
gentamicin. G418 is toxic to both prokaryotic and eukaryotic
cells. It is used primarily for the selection of eukaryotic
expression vectors bearing the related resistance genes.
Recommended concentration varies by cell type; in mammalian
cells, 400 mg/L is recommended for initial selection, and then
reduced to 200 mg/L for culture maintenance.
Ships on ice. Store refrigerated at 2 to 8°C
G418 is also known by the trademark Geneticin®, as registered to
Invitrogen Corporation.
G418 sulfate, an aminoglycoside antibiotic, is related to
gentamicin. G418 is toxic to both prokaryotic and eukaryotic
cells. It is used primarily for the selection of eukaryotic
expression vectors bearing the related resistance genes.
Recommended concentration varies by cell type.
Sterile-filtered
Ships on ice. Store refrigerated at 2 to 8°C
G418 is also known by the trademark Geneticin®, as registered to
Invitrogen Corporation.
Gentamicin kills Gram-negative and Gram-positive bacteria, and
mycoplasma. It works by binding to microbial ribosomes and
preventing the initiation of protein synthesis. Gentamicin is
very stable in solution and lasts many days in culture. It is
generally more effective than streptomycin. This product should
be used with caution, as high concentrations can retard cell
growth. Recommended concentration in cell culture is 50 mg/L.
Gentamicin kills Gram-negative and Gram-positive bacteria, and
mycoplasma. It works by binding to microbial ribosomes and
preventing the initiation of protein synthesis. Recommended
dosing in media is 1 mL/L.
Sterile-filtered
Ships on ice. Store refrigerated at 2 to 8°C
Hygromycin B, an aminoglycoside antibiotic, acts against
bacteria, fungi and higher eucaryotes by inhibiting protein
synthesis. Originally developed as an antihelminthic, hygromycin
B is used in the research laboratory primarily for the selection
and maintenance of cells that carry the hygromycin resistance
gene.
Hygromycin B, an aminoglycoside antibiotic, acts against
bacteria, fungi and higher eukaryotes by inhibiting protein
synthesis. Originally developed as an antihelminthic, hygromycin
B is used in the research laboratory primarily for the selection
and maintenance of cells that carry the hygromycin resistance
gene.
Kanamycin, an aminoglycoside antibiotic, acts against bacteria,
fungi and higher eucaryotes by inhibiting protein synthesis.
Kanamycin is used in the research laboratory primarily for the
selection and maintenance of cells that carry the kanamycin
resistance gene.
Mitomycin C is an anti-neoplastic antibiotic, DNA inter-strand,
cross-linking alkylating agent that targets guanine nucleoside in
the sequence 5 CpG-3 . It produces oxygen radicals and is
preferentially toxic to hypoxic cells. It is used to generate
mitotically inactive feeder cells used in cell culture systems,
such as mitotically inactive fibroblast used in embryonic stem
cell (ESC) systems. Soluble in methanol and water.
Neomycin Sulfate is an aminoglycoside antibiotic that inhibits
translation by binding to the small subunit of prokaryotic
ribosomes. Soluble in water. Protect from light and moisture.
Penicillin G was one of the first and safest antibiotics used in
cell culture. It acts by inhibiting bacterial cell wall
synthesis. Traditionally it has been used in combination with
streptomycin for broader coverage of Gram-negative and
Gram-positive bacteria. Due to its long-time use in laboratories,
the newer combination of Penicillin G and Gentamicin (Pen-Gent,
catalog #400-129) is more often recommended to combat organisms
resistant to streptomycin. Recommended concentration is 100
units/mL. Penicillin G has no activity against fungi.
Penicillin:streptomycin (pen:strep) solution is a standard
antibiotic mixture used against a wide variety of Gram-positive
and Gram-negative bacterial organisms. It has no activity against
fungi. This product is useful both as a preventive agent and as a
treatment for bacterially contaminated cultures. However,
organisms resistant to one or both antibiotics are becoming more
common in cell culture laboratories. Investigators need to be
alert to this possibility and consider other antibiotics.
Puromycin acts against bacteria, fungi and higher eukaryotes by
inhibiting protein synthesis. Puromycin is used in the research
laboratory primarily for the selection and maintenance of cells
that carry the puromycin resistance gene.
A macrocyclic triene antibiotic that binds to and inhibits the
molecular target of rapamycin (mTOR). It
forms a complex with FKBP12 that binds to and inhibits the
molecular target of rapamycin (mTOR).
Rapamycin is a potent immunosuppressant and has anticancer
activity.
Ships on dry ice. Store frozen at -20 to -10°C
Protect from light and moisture.
Streptomycin is one of the oldest and safest antibiotics for use
in cell culture to control many Gram-negative and Gram-positive
bacteria. It inhibits bacterial protein synthesis, and is
commonly used in combination with penicillin G for a broader
spectrum of activity. It has no effect on fungi.
