This perfect blend of trypsin (0.25%) and EDTA (0.53mM) is combined in calcium-free and magnesium-free Hanks Balanced Salt Solution along with phenol red. It has been shown to be a safe and effective means of dissociating a wide variety of cells in culture.

Sterile-filtered
Porcine parvovirus screened
Ships on dry ice. Store frozen at -20 to -10°C

Puromycin acts against bacteria, fungi and higher eukaryotes by inhibiting protein synthesis. Puromycin is used in the research laboratory primarily for the selection and maintenance of cells that carry the puromycin resistance gene.

Ships on dry ice. Store frozen at -20 to -10°C

L-Glutamine:Penicillin:Streptomycin is a standard combination of penicillin and streptomycin plus the essential amino acid L-glutamine. This stabilized formulation (with L-alanyl-L-glutamine) prevents the degradation and cytotoxicity known to be associated with the monopeptide L-glutamine, markedly increasing shelf life. It is furnished in a convenient 100X concentration to simplify media supplementation.

Sterile-filtered
Ships on dry ice. Store frozen at -20 to -10°C

Kanamycin, an aminoglycoside antibiotic, acts against bacteria, fungi and higher eucaryotes by inhibiting protein synthesis. Kanamycin is used in the research laboratory primarily for the selection and maintenance of cells that carry the kanamycin resistance gene.

Ships at ambient. Store dry at 15 to 30°C

Gentamicin kills Gram-negative and Gram-positive bacteria, and mycoplasma. It works by binding to microbial ribosomes and preventing the initiation of protein synthesis. Gentamicin is very stable in solution and lasts many days in culture. It is generally more effective than streptomycin. This product should be used with caution, as high concentrations can retard cell growth. Recommended concentration in cell culture is 50 mg/L.

Ships on ice. Store refrigerated at 2 to 8°C 

G418 sulfate, an aminoglycoside antibiotic, is related to gentamicin. G418 is toxic to both prokaryotic and eukaryotic cells. It is used primarily for the selection of eukaryotic expression vectors bearing the related resistance genes. Recommended concentration varies by cell type; in mammalian cells, 400 mg/L is recommended for initial selection, and then reduced to 200 mg/L for culture maintenance.

Ships on ice. Store refrigerated at 2 to 8°C
G418 is also known by the trademark Geneticin®, as registered to Invitrogen Corporation. 

This beta-lactam antibiotic is an aminopenicillin. Like penicillin, it inhibits bacterial cell wall synthesis by preventing peptidoglycan cross-linking and murein assembly. The presence of an amino group confers greater efficacy against Gram-negative bacteria as compared to penicillin. Ampicillin is commonly used in molecular biology to assay for gene uptake (e.g., plasmid vectors carrying the ampicillin resistance gene).

Ships on ice. Store refrigerated at 2 to 8°C

Amphotericin B is one of the earliest compounds designed to kill fungi. It was developed to help treat fungal infections intravenously. It has since been found to help eliminate fungal contamination in cell cultures as well. Low doses (0.5 to 2.0 ug/mL) are recommended as it is toxic to other eukaryotic cells at higher concentrations.

Final concentration of Amphotericin B is undiluted.

Sterile-filtered
Ships on dry ice. Store frozen at -20 to -10°C
FUNGIZONE® is a registered trademark of E.R. Squibb

Neomycin Sulfate is an aminoglycoside antibiotic that inhibits translation by binding to the small subunit of prokaryotic ribosomes. Soluble in water. Protect from light and moisture.

Ships at ambient. Store dry at 15 to 30°C