ITS (Insulin, Transferrin, Selenium) is a chemically defined
supplement for a variety of cell culture applications. ITS
supplement contains components which are required for optimal
performance of serum-free media. Supplementation of ITS to
different conventional nutrient media substantially reduces the
FBS requirement for routine maintenance of many cell lines.
Components: Contains 1.0 mg/ml insulin from bovine pancreas, 0.55
mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml
sodium selenite.
This diagnostic-grade product is a sterile, aqueous solution
containing 20% human albumin (w/v). It is prepared by
cold-alcohol fractionation from pooled human plasma obtained from
venous blood. The USP Pharmacopeia requires that all
fractionators add sodium caprylate and sodium tryptophanate as a
preservative.
Store frozen at -20 to -10°C
Please inquire about therapeutic grade material, if required by
application
Comparable to B27®, Gem21 NeuroPlex ™ is a chemically defined,
serum-free supplement for use in the culture of neural cells and
to promote the differentiation of embryonic stem cells into
pancreatic and neuronal lines. When used in conjunction with our
NeuroPlex ™ serum-free neuronal medium, this product obviates the
need for co-culture with astrocyte feeder cells. Supplied as a
50X concentrate.
Sterile filtered
Ships on dry ice. Store frozen at -20 to -10°C
B27® is a registered trademark of Invitrogen Corporation
This diagnostic-grade product is a sterile, aqueous solution
containing 25% human albumin (w/v). It is prepared by
cold-alcohol fractionation from pooled human plasma obtained from
venous blood. The USP Pharmacopeia requires that all
fractionators add sodium caprylate and sodium tryptophanate as a
preservative.
Store frozen at -20 to -10°C
Please inquire about therapeutic grade material, if required by
application.
Comparable to B27®, Gem21 NeuroPlex™ is a chemically defined,
serum-free supplement with vitamin A removed. Removing Vitamin A
makes this supplement optimal for growth of embryonic and adult
neural stem cells and neural precursors. When used in conjunction
with our NeuroPlex™ serum-free neuronal medium, this product
obviates the need for co-culture with astrocyte feeder cells.
Supplied as a 50X concentrate.
Sterile-filtered
Ships on dry ice. Store frozen at -20 to -10°C
B27® is a registered trademark of Invitrogen Corporation
Comparable to B27®, Gem21 NeuroPlex™ is a chemically defined,
serum-free supplement with the antioxidants removed. It is
intended for use in the culture of neuronal cells and to promote
the differentiation of embryonic stem cells into pancreatic and
neuronal lines, and the study of free radicals.
When used in conjunction with our NeuroPlex™ serum-free neuronal
medium, this product obviates the need for co-culture with
astrocyte feeder cells. Supplied as a 50X concentrate.
G5 NeuroPlex ™ is a chemically defined, serum-free supplement
intended for use in the culture of astrocytic glial cells.
Supplied as a 100X concentrate.
Sterile filtered
Ships on dry ice. Store frozen at -20 to -10°C
N2 NeuroPlex ™ is a chemically defined, serum-free supplement
intended for use in the culture of post-mitotic neuronal cells.
Supplied as a 100X concentrate.
Sterile filtered
Ships on dry ice. Store frozen at -20 to -10°C
Insulin is a polypeptide hormone produced in the pancreatic
islets of Langerhans that has a molecular weight of ~5800 Da.
Insulin primarily regulates the uptake and storage of glucose in
cellular systems. It also plays a related role in the metabolism
of certain amino acids and fatty acids. Bovine insulin is
remarkably similar to human insulin, differing in molecular
structure by only three amino acid residues.
Cell culture tested
Ships ambient. Store frozen at -20 to -10°C
Insulin is a polypeptide hormone produced in the pancreatic
islets of Langerhans that has a molecular weight of ~5800 Da.
Insulin primarily regulates the uptake and storage of glucose in
cellular systems. It also plays a related role in the metabolism
of certain amino acids and fatty acids. Bovine insulin is
remarkably similar to human insulin, differing in molecular
structure by only three amino acid residues.
Cell culture tested
Ships ambient. Store frozen at -20 to -10°C
No-Worries ™ bovine pituitary extract (BPE) comes from
USDA-approved sources in New Zealand. Geographically isolated,
New Zealand livestock is internationally recognized as free from
bovine spongiform encephalopathy (BSE, mad-cow disease) and,
unlike Australia, has no history of bluetongue disease in cattle.
Gemini’s chicken embryo extract is prepared from 9-12 day old
eggs. It is processed as a 50% extract in phosphate-buffered
saline that is supplemented with antibiotic:antimycotic. The
liquid extract is sterile filtered to 0.2 µm and lyophilized in
10mL aliquots. The sterility of this lyophilized powder cannot be
guaranteed and should be sterile filtered once more after
reconstitution.
Lyophilized for longest shelf life
Ships at ambient. Store refrigerated at 2 to 8°C.
Gemini’s standard grade BSA is a highly purified, lyophilized
powder. Its characteristics include minimum 98% albumin, pH
adjusted to 7.0, caprylate-free, and low conductivity. It is used
in bacterial media, enzyme systems, and as a protein base.
Gemini’s protease-free BSA is a highly purified, lyophilized
powder. This product is processed to provide a minimum of 98%
albumin and to be essentially free of detectable protease. The pH
is adjusted to 7.0. It is used in diagnostic reagent
manufacturing and as a model protein for blood banking reagents.
Gemini’s Cohn fraction BSA is made by a modification of the
original cold-ethanol plasma fractionation process developed in
1939 by Professor Edwin Cohn. Its characteristics include a
minimum 96% albumin, with a pH of 7.0 – 7.4. This product is
suitable as a growth nutrient in tissue culture media, for
increasing the viscosity and protein levels in certain antibiotic
assay media, to bind inhibitors in growth media, and as a
molecular-weight standard.
Gemini’s low-endotoxin BSA is a highly purified, lyophilized
powder. This product is processed to provide a minimum of 98%
albumin and to be essentially free of protease, IgG and
non-esterified fatty acids. Endotoxin content is typically less
than 0.5 EU/mg. The pH is adjusted to 7.0. It is ideal for most
cell culture applications and assays that require low endotoxin
levels.
Comparable to AlbuMAX®, our serum-replacement grade BSA is a
highly purified, lyophilized powder. Formulated to optimize the
product’s lipid profile for growth promotion, this lipid-enriched
BSA is processed to provide a minimum of 98% albumin and to be
essentially free of IgG. This low-endotoxin preparation is
intended for serum-free and serum-reduced cell culture
applications.
Gemini’s low-IgG BSA is a highly purified, lyophilized powder.
This product is processed to provide a minimum of 98% albumin and
to be essentially free of immunoglobulin. The pH is adjusted to
7.0. It is used as a blocking reagent in EIA, RIA and ELISA
systems, where IgG may interfere with the application.
Gemini’s molecular biology grade BSA is a highly purified,
lyophilized powder. This product is processed to provide a
minimum of 98% albumin and to be essentially free of both
protease and nuclease activity. The pH is adjusted to 7.0. It is
commonly used in PCR and other molecular biology applications.
Gemini’s fatty acid-free BSA is a highly purified, lyophilized
powder. This product is processed to provide a minimum of 98%
albumin and to be essentially free of non-esterified fatty acids.
The pH is adjusted to 7.0. It is used as a blocking reagent in
EIA, RIA and ELISA systems, where serum fatty acids may interfere
with the application.
