Gemini Bio-Products stores product for customers free of additional charge for up to one year, provided a Purchase Order has been received. After one year, customers are billed a $3 per 500 mL bottle, $1 per 100 mL bottle fee for each additional month that their product is stored.
We do not add this antibiotic to serum during manufacturing. Tetracycline is an antibiotic which is often used to treat animals prior to slaughter. Although there should be sufficient time between the last dose of antibiotic and slaughter for the drug to metabolize, this is not always the case and the serum may contain residual amounts of tetracycline.
Gemini tests each lot of fetal bovine serum and Fetalplex™ for tetracycline and we report these results on the product’s respective Certificate of Analysis.
All GemCell™ products are strictly of US origin. Gemini Bio-Products procures its serum from USDA-approved and inspected slaughter houses, both inside and outside the continental United States. Gemini Bio-Products will supply a Certificate of Origin for any sera product upon request.
Newborn Calf Serum is derived from bovine calves that are less than ten days old, but are more than one day old. These calves have suckled their mothers and the serum is not colostrum-free. Bovine Calf Serum is derived from bovine calves that are more than ten days, but less than eighteen months old.
Although it is impossible to claim stability data for every possible application and use for human serum AB, we conservatively estimate that if this product is stored properly at -20ºC, it can be used for 5 years from the date of manufacture without any decrease in product performance. Storage at any other temperature may affect results.
Gemini Bio-Products goes to great lengths to ensure that our human sera are the most consistent products commercially available. Although our human serum is manufactured using raw material from congruous donor pools and according to time-tested protocols, it is possible to perceive differences in the physical appearance of this product from lot-to-lot. This phenomenon can be largely attributed to variation in diet amongst human beings (particularly with respect to dietary fats).
Due to a diet higher in fats and oils, human serum typically contains higher levels of lipids than serum from other sources. Even after sterile filtration through 0.1mm filters, particles remain suspended in solution that can aggregate and precipitate as a result of subsequent freezing, thawing and/or heat-inactivation. It is for this reason that we suggest minimizing such temperature changes. The presence of particulate matter does not indicate contamination of any sort, and does not negatively impact the serum’s performance.
Products can be reserved at the time samples are sent out by requesting the reserve quantity at the time of sampling. Customers should request the reserve quantity through their sales representative or by calling Gemini’s Customer Service Team at 1-800-543-6464.
Cell culture contamination has been a major concern for researchers since cell culture was introduced in the early 1900’s. One particular villain to the field of in vitro research has been endotoxin. But what do we really know about it?
You may be familiar with the terms “traceability” and “origin” in reference to fetal bovine serum (FBS), but how do these topics really impact the use of serum in cell culture? “Origin” is simply the country in which the raw material was collected. “Traceability” refers to the documentation roadmap that connects serum origin to final manufacturing and distribution.
Albumin is one of the most extensively used proteins in biological research today. It acts as a powerful antioxidant in cell culture. It binds, sequesters and stabilizes a variety of molecular species which are often unstable. This acidic, soluble protein has both high- affinity and secondary binding sites, optimizing the roles that fatty acids, metals, disulfides, and other molecules play in cellular metabolism.
With all of the choices for bovine serum albumin (BSA) available, you may be wondering what the differences are and which product is right for your application.