How could your cells be optimized for Gibco FBS – unless you have
been using the same lot all these years? Every time the lot
changes, you have a new serum. Hundreds of different
fetuses from different collection sites, go into making each new
lot – - – the same as with Gemini. Therefore every lot is a
bit different from every other lot. If you look at the
specs for the serum types, Gemini’s specs for each grade of FBS
is much tighter than Gibco’s – so there would be less lot to lot
variations with the Gemini than with the Gibco FBS.
ITS (Insulin, Transferrin, Selenium) is a chemically defined
supplement for a variety of cell culture applications. ITS
supplement contains components which are required for optimal
performance of serum-free media. Supplementation of ITS to
different conventional nutrient media substantially reduces the
FBS requirement for routine maintenance of many cell lines.
Components: Contains 1.0 mg/ml insulin from bovine pancreas, 0.55
mg/ml human transferrin (substantially iron-free), and 0.5 µg/ml
sodium selenite.
