How could your cells be optimized for Gibco FBS – unless you have been using the same lot all these years? Every time the lot changes, you have a new serum. Hundreds of different fetuses from different collection sites, go into making each new lot – - – the same as with Gemini. Therefore every lot is a bit different from every other lot. If you look at the specs for the serum types, Gemini’s specs for each grade of FBS is much tighter than Gibco’s – so there would be less lot to lot variations with the Gemini than with the Gibco FBS.
ITS (Insulin, Transferrin, Selenium) is a chemically defined supplement for a variety of cell culture applications. ITS supplement contains components which are required for optimal performance of serum-free media. Supplementation of ITS to different conventional nutrient media substantially reduces the FBS requirement for routine maintenance of many cell lines.
Components: Contains 1.0 mg/ml insulin from bovine pancreas, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 µg/ml sodium selenite.