hPL Frequently Asked Questions
What is the advantage of hPL over FBS or Human AB Serum?
hPL offers better lot-to-lot consistency. In addition, lower concentrations can be used than with sera.
What is the shelf life for the hPL?
When stored at -20°C, hPL can be used up to 29 months after the date of manufacturing.
Does the hPL contain heparin or any clotting factors?
There are no clotting factors in the hPL. No heparin is used and fibrinogen has been removed to minimize the risk of clotting.
What is the typical concentration used for hPL in complete media?
Studies have shown that a concentration as low as 2% can be used in complete media. However, this may depend on your cell type and needs to be validated.
What is the difference between the research-grade and cGMP-grade hPL?
Both grades are manufactured under the same stringent conditions and protocols. However, the cGMP-grade hPL undergoes more rigorous testing.
How should the hPL be handled after receiving it?
Store the hPL at -20°C upon arrival. Before using, aliquot the bottle to the volume needed for each use. We do not recommend thawing the product more than two times.
How long does the product last once added to media?
When stored properly at 4°C, we recommend using the complete media within 2-3 weeks.
What is the difference between BenchMark™ and GemCell™ hPL?
These two versions are manufactured under the same conditions. However, BenchMark™ has less extensive testing before release. The pH, osmolality, growth verification, and total protein content are not tested with the BenchMark™ hPL. Still, it is almost guaranteed to yield the same testing results if it were tested. This option is more viable for labs working with RUO products or that want to try hPL and do not have the initial budget to purchase one of the GemCell™ products.