Should I culture with the Petaka G3™ in an upright or horizontal configuration?


Suspension cells must be cultured in the Petaka G3™ in a horizontal configuration, similar to a flask because these cells will clump on the bottom when the Petaka G3™ is cultured upright. After adherent cells attach, (typically 1-2 hours), the Petaka G3™ may be left in the horizontal position or it may be switched to an upright position for the duration of the culture. However, when the Petaka G3™ is kept “horizontal” it is strongly advised to elevate the top end of the Petaka G3™ slightly higher than the bottom (5°-15°) to limit the amount of medium that may enter the capillary breakers at the top of the Petaka G3™.