Nourish, improve and protect your cell cultures with amino acids, antibiotics and antimycotics, attachment factors, cell dissociation reagents, culture supplements, albumins, and recombinant proteins.
L-Glutamine is an essential amino acid commonly used in cell culture. However, it is notably unstable in liquid media, spontaneously breaking down to release cytotoxic amines. This stabilized compound (L-alanyl-L-glutamine) prevents degradation and cytotoxicity, markedly increasing shelf life.
Ships at ambient. Store dry at 15 to 30°C
CAS Number 39537-23-0
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L-Glutamine is an essential amino acid commonly used in cell culture. However, it is notably unstable in liquid media, spontaneously breaking down to release cytotoxic amines. This stabilized compound (L-alanyl-L-glutamine) prevents degradation and cytotoxicity, markedly increasing shelf life. This solution is prepared at a convenient 200mM (100x) concentration.
Sterile-filtered
Ships on dry ice. Store frozen at -20 to -10°C
CAS Number 39537-23-0
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L-Glutamine:Penicillin:Streptomycin is a standard combination of penicillin and streptomycin plus the essential amino acid L-glutamine. This stabilized formulation (with L-alanyl-L-glutamine) prevents the degradation and cytotoxicity known to be associated with the monopeptide L-glutamine, markedly increasing shelf life. It is furnished in a convenient 100X concentration to simplify media supplementation.
Sterile-filtered
Ships on dry ice. Store frozen at -20 to -10°C
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Amphotericin B is one of the earliest compounds designed to kill fungi. It was developed to help treat fungal infections intravenously. It has since been found to help eliminate fungal contamination in cell cultures as well. Low doses (0.5 to 2.0 ug/mL) are recommended as it is toxic to other eukaryotic cells at higher concentrations.
Final concentration of Amphotericin B is undiluted, 250ug/mL.
Sterile-filtered
Ships on dry ice. Store frozen at -20 to -10°C
FUNGIZONE® is a registered trademark of E.R. Squibb
CAS Number 1397-89-3
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This beta-lactam antibiotic is an aminopenicillin. Like penicillin, it inhibits bacterial cell wall synthesis by preventing peptidoglycan cross-linking and murein assembly. The presence of an amino group confers greater efficacy against Gram-negative bacteria as compared to penicillin. Ampicillin is commonly used in molecular biology to assay for gene uptake (e.g., plasmid vectors carrying the ampicillin resistance gene).
Ships on ice. Store refrigerated at 2 to 8°C
CAS Number 7177-48-2
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Gemini’s antibiotic:antimycotic (anti:anti) solution is for general prophylactic use. Dilute 100-fold for use in media (10mL/L). This product is useful as a broad spectrum anti-bacterial and anti-fungal agent. This product is not recommended for cultures with established fungal contamination due to the low concentration of amphotericin B. See Amphotericin B for elimination of fungal contamination in culture.
10,000 units/mL penicillin G, 10mg/mL streptomycin sulfate, 25µg/mL amphotericin B with 8.5 g/L NaCI
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Plasma fibronectin, also called cold-insoluble globulin or CIG, is a component of blood plasma produced in the liver. It binds extracellular matrix components such as collagen and fibrin and is used as an attachment factor in cell culture. This product is derived from human plasma.
Stabilized in buffered glycerol
Sterile filtered
Ships on ice. Store refrigerated at 2 to 8°C
CAS Number 86088-83-7
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Gemini’s standard grade BSA is a highly purified, lyophilized powder. Its characteristics include minimum 98% albumin, pH adjusted to 7.0, caprylate-free, and low conductivity. It is used in bacterial media, enzyme systems, and as a protein base.
Ships ambient. Store refrigerated at 2 to 8°C
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Gemini’s protease-free BSA is a highly purified, lyophilized powder. This product is processed to provide a minimum of 98% albumin and to be essentially free of detectable protease. The pH is adjusted to 7.0. It is used in diagnostic reagent manufacturing and as a model protein for blood banking reagents.
Ships ambient. Store refrigerated at 2 to 8°C
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Gemini’s low-endotoxin BSA is a highly purified, lyophilized powder. This product is processed to provide a minimum of 98% albumin and to be essentially free of protease, IgG and non-esterified fatty acids. Endotoxin content is typically less than 0.5 EU/mg. The pH is adjusted to 7.0. It is ideal for most cell culture applications and assays that require low endotoxin levels.
Ships ambient. Store refrigerated at 2 to 8°C
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This perfect blend of trypsin (0.05%) and EDTA (0.53mM) is combined in calcium-free and magnesium-free Hanks Balanced Salt Solution along with phenol red. It has been shown to be a safe and effective means of dissociating a wide variety of cells in culture.
Sterile-filtered
Porcine parvovirus screened
Ships on dry ice. Store frozen at -20 to -10°C
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This perfect blend of trypsin (0.25%) and EDTA (0.53mM) is combined in calcium-free and magnesium-free Hanks Balanced Salt Solution along with phenol red. It has been shown to be a safe and effective means of dissociating a wide variety of cells in culture.
Sterile-filtered
Porcine parvovirus screened
Ships on dry ice. Store frozen at -20 to -10°C
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Comparable to B27®, Gem21 NeuroPlex™ without Insulin is a chemically defined, serum-free supplement with insulin removed. This supplement is used to support the growth of CNS neurons and other cells in applications where the presence of insulin causes interference. Supplied as a 50X concentrate.
Sterile-filtered
Ships on dry ice. Store frozen at -20 to -10°C
B27® is a registered trademark of Invitrogen Corporation
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This product is an aqueous solution containing 10 mg/mL of human recombination insulin. Insulin regulates uptake, utilization and storage of glucose, amino acids and fatty acids in cells and inhibits decomposition of glycogen, proteins and lipids. Recommended for use in cell culture applications at 0.5 to 1 mL per liter of medium, but should be optimized for each process.
Ships Dry Ice. Store frozen at -20 to -10°C
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Gemini’s chicken embryo extract is prepared from 9-12 day old eggs collected from registered flocks and found to be free from clinical signs of notifiable diseases.
It is processed as a 50% extract in phosphate-buffered saline that is supplemented with 100IU/mL Penicillin, 100 ug/mL Streptomycin and 2.5ug/mL Amphotericin. The liquid extract is sterile filtered to 0.2 µm and lyophilized in 10mL aliquots. The sterility of this lyophilized powder cannot be guaranteed and should be sterile filtered once more after reconstitution.
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Basic fibroblast growth factor is also known as bFGF, FGF2 or FGF-beta. It is used to support the culture of embryonic stem cells. This carrier-free product is a recombinant human protein expressed in E. coli.
Ships ambient. Store frozen at -20 to -10°C
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Epidermal growth factor, or EGF, plays and important role in the regulation of cell growth, proliferation and differentiation. This carrier-free product is a recombinant human protein expressed in E. coli.
Ships ambient. Store frozen at -20 to -10C
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Interleukin-3 is a cytokine produced by activated T-cells and mast cells and is active on a broad range of hematopoietic cells and in the nervous system. It appears to be important in several chronic inflammatory diseases. This product is a recombinant protein produced in E. coli, and is used to grow/differentiate multiple types of blood cells from stem cells and is required to culture several leukemic cell lines (ex. NFS-60 line).
Ships ambient. Store frozen at -20 to -10C.
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Continuum™ Transfection Reagent is a powerful and versatile DNA and siRNA transfection reagent for the transfection of primary cells and other difficult-to-transfect cells. Gemini’s Continuum™ has been shown to transfect the broadest spectrum of adherent and suspension cells. Continuum™ is incredibly efficient, requiring half the amount when compared to other offerings.
A mixture of cationic polymers and lipids, with 100% animal origin free components
Ships ambient. Store refrigerated at 2 to 8°C
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