A novel solid tumor model in the Petaka G3: a simple, progressive, in-vitro model of the harsh tumor microenvironment and its implications in metastasis research
We present a model of chronic, progressive tumor hypoxia and nutritional restriction that is not easily replicated in any other device, and which establishes the minimum oxygen consumption of these cells in a manner similar to their in-vivo condition. With this system, we provide for much longer chronic treatment than is possible in an open culture system like flasks. In addition, we can easily multi-sample from the Petaka over weeks, so researchers can serially measure biomarkers of interest without disturbing the hypoxia treatment, and without glove boxes. We will demonstrate improved cell viability and recovery at 30 and 45-days compared to open systems and demonstrate how to measure pO2 and doubling time to track determinants of treatment resistance and compare them to recovery outcomes.